use of response surface methodology analysis for xanthan biopolymer production by xanthomonas campestris: focus on agitation rate, carbon source, and temperature

the current study is an attempt to contribute for efficient and cost-effective substrates for xanthan gum production. in this context, the sugar cane molasses wastes can be used as a cheap substrate for xanthan gum production. xanthan biopolymer production by a novel xanthomonas campestris strain ibrc-m 10644 was optimized with statistical approaches. based on the results of response surface methodology (rsm) with central composite design (ccd) technique, a second-order polynomial model was developed and evaluated the effects of variables on the maximum xanthan production. agitation rate (x1: 200-500 rpm), sugar cane molasses concentration (x2: 30-90 g/l) and operation temperature (x3: 25-35 °c) were the factors investigated. the optimal conditions for maximum yield of xanthan production were derived from agitation rate 500 rpm, carbon source concentration 65 g/l and operation temperature 30°c. under these conditions, xanthan and biomass production were found to be 16.03 g/l and 1.37 g/l, respectively. our results signify that the sugar cane molasses can be used as a cheap substrate for xanthan biopolymer production.